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. 2018 Feb 12;8:2797. doi: 10.1038/s41598-018-21143-0

Figure 6.

Figure 6

Dose-dependent firefly luciferase activity after recMgIFN-γ induction in a bank vole reporter cell line. Bioluminescence assay on the reporter cell line BVK-LucA expressing the firefly luciferase gene under the control of a GAS promoter element. Cells were either left untreated (ctrl, not shown) or treated with the control protein LEA (200 ng/ml); mouse IFN-γ (200 ng/ml) or recMgIFN-γ (200 to 0.00002 ng/ml). The reported bioluminescence values are relative to untreated cells. Mean ± SEM (n = 4 experiments, each with 3 replicates). Statistical analysis: One-way Anova (F(9, 30) = 76,16, p =  < 0.0001) followed by Dunnett’s multiple comparisons test (all against the LEA control protein). ***p = 0.0002, ****p ≤ 0.0001, relative to the control protein LEA. Note that only statistically significant (p ≤ 0.05) differences were depicted in the figure.