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. 2017 Oct;187(10):2273–2287. doi: 10.1016/j.ajpath.2017.06.009

Figure 2.

Figure 2

PDGFRα expression and activity in hepatic stellate cells. A: HHSteC and LX-2 cells show differential expression of PDGFRα at baseline and a corresponding increase in phosphorylated receptor after 10 ng/mL PDGF-BB treatment for 2 minutes (m). B: HHSteCs stimulated with 10 ng/mL PDGF-AA for the indicated time periods show increased PDGFRα phosphorylation at Y742, Y849, and Y572/574. Associated downstream signaling mediators Akt and protein kinase C (PKC) also showed increased phosphorylation in response to PDGF-AA treatment. Arrowheads indicate the correct molecular weight band. C: Direct binding of human antibodies to immobilized (coated) human PDGFRα or PDGFRβ in an enzyme-linked immunosorbent assay. Antibody 2C5 serves as a positive control anti-PDGFRβ antibody. Ponceau staining is included as a loading control. h, hours.