Figure 8.

Densitometric quantification of olaratumab-mediated changes in protein phosphorylation in HHSteCs at baseline. A: Densitometry of key phosphorylated protein changes from representative Western blots using pooled samples from three technical replicates for each time point shown in Figure 7. Values for each phosphorylated protein signal are normalized to total protein levels and shown as the ratio of the signal of olaratumab-treated HHSteCs/IgG-treated HHSteCs. B: c-Abl–phosphorylated and total proteins are represented separately to highlight the increase in both total and phosphorylated proteins. The dashed line represents the value at which the relative phosphorylated protein signals of olaratumab-treated HHSteCs and IgG-treated HHSteCs are equal. h, hours; m, minutes.