Fig. 4.
Application of q2PISH to assess the impact of different FBS on biomarker expression. Fetal bovine serum (FBS) received from two different suppliers (A and B) was tested for the expression of a subset of biomarkers for two different cell lines (I and II) in culture. a qPISH expression data per cell was subjected to paired student t-test, which did not reveal any significant difference in gene expression between FBS of supplier A an B for the genes analyzed. Arbitrary units (a.u.) are reflected on the y-axis. b Nuclear cell count at the experimental endpoint indicated a significant reduction in cell survival for cells cultured with FBS from supplier B. c Qualitative assessment of gene expression post quantification as shown here exemplary for Col1a1 expression by cell line I clearly indicates heterogeneity of the culture, showing cells with high (red arrow), medium (green arrow) and low (blue arrow) level of Col1a1 expression for FBS from both suppliers. qPISH expression data per cell and cell count was subjected to unpaired student’s t-test with no significant difference (ns) indicating a p value of p > 0.05, **indicating a p value of p ≤ 0.01 and ***indicating a p value of p ≤ 0.001. Error bar is indicating the standard error