Fig. 3.

E2 secretion and CYP19 protein expression in JEG-3 (A, B), BeWo (C, D) and syncBeWo (E, F) mono- and co-cultures with H295R. The protein levels of CYP19 (50 kDa), were densitometrically scanned and normalised against the β-actin (42 kDa) signal. ELISA and Western blotting experiments were independently performed and repeated three times (n = 3). The data are plotted as the mean ± S.D. Statistical significant differences between time of culture and mono versus coculture are indicated with different letters (P < 0.05), the same letters indicating no significant differences