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. 2018 Feb 12;38(5):e00409-17. doi: 10.1128/MCB.00409-17

FIG 2.

FIG 2

TAB1-mediated autoactivation is impaired in p38α(T185G) compared to wtp38α. (A) Western blot analysis of the products of an in vitro kinase assay performed with wtp38α or p38α(T185G) in the absence or in the presence of TAB1(384–412) peptide at 30 and 60 min. The T185G substitution impedes autoactivation. (C) Western blot analysis of in vitro activation of p38α(T185G) and wtp38α by upstream kinase MKK6dd. (B and D) Western blot analysis of an in vitro kinase assay of dually phosphorylated wtp38α or p38α(T185G) with ATF2 (B) and TAB1 (D), two known substrates of p38α. The mutant p38α(T185G) is catalytically competent.