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The activity of NagB was measured as a function of HPr concentration, 0 to 1.8 μM, with 3 mM GlcN-6P in the presence of 0.3 μM NanE (■) or 0.05 μM U-PII (○) and in the absence of both proteins and the presence of the effector GlcNAc-6P at 0.2 mM (●). Synergy of HPr and NanE was observed at concentrations of HPr in excess of 0.8 μM.
