FIG 5.

Growth of the WT, ΔcvsS, ΔcvsR, and ΔcvsRc strains on NB supplemented with 5 mM CaCl₂ and CW after 16 h (A) and 3 days (B) of growth. Fluorescence of the bacterial strains under UV light indicates production of cellulose. Bacterial strains were grown to stationary phase in KB medium and resuspended at an OD₆₀₀ of 0.3 in NB medium, and then 5 μl of each culture was spotted onto the appropriate medium. The plate is representative of assays that were performed three times.