FIG 1.

Dual intranasal inoculation with NL/09wt and NL/09var viruses resulted in high levels of reassortment. Ferrets were inoculated intranasally with a 1:1 mixture of NL/09wt and NL/09var viruses in a 40-μl volume. The dose used comprised 1 × 10⁵ TCID₅₀ of each virus. Nasal swabs were collected and virus therein analyzed to generate the data shown. (A) Viral genotypes of 17 to 21 plaque isolates per sample are shown in table format, where each row represents a viral isolate and each of the eight columns represents a viral gene segment in the order PB2, PB1, PA, HA, NP, NA, M, and NS. Red bars indicate segments derived from the NL/09wt parental virus, and turquoise bars show segments derived from the NL/09var virus. White bars indicate segments where the genotyping result was not clear. Day postinoculation (p.i.) is shown at the top, and the ferret from which swabs were collected is indicated at the left. (B) Infectious titers of nasal swab samples are plotted as a function of time postinoculation. (C) The diversity of viral gene constellations detected is represented for each ferret, with days p.i. indicated in grayscale. Each sample's Simpson index value was converted to a corresponding Hill number (Hill's N₂) to derive its effective diversity.