FIG 2.

Dual intranasal inoculation with Pan/99wt and Pan/99var viruses resulted in high levels of reassortment. Ferrets were inoculated intranasally with a 1:1 mixture of Pan/99wt and Pan/99var viruses in a 40-μl volume. The dose used comprised 1 × 10⁵ TCID₅₀ of each virus. Nasal swabs were collected and virus therein analyzed to generate the data shown. (A) Viral genotypes of 17 to 21 plaque isolates per sample are shown in table format, as described in the legend to Fig. 1. Red bars indicate segments derived from the Pan/99wt virus, and turquoise bars show segments derived from the Pan/99var virus. White bars indicate segments where the genotyping result was not clear. (B) Infectious titers of nasal swab samples are plotted as a function of time postinoculation. (C) The diversity of viral gene constellations detected is represented for each ferret, with days p.i. in grayscale. Each sample's Simpson index value was converted to a corresponding Hill number (Hill's N₂) to derive its effective diversity.