FIG 8.

Separate inoculations of Pan/99wt and Pan/99 viruses by the intranasal and intratracheal routes yielded moderate mixing but minimal reassortment in the throat. Ferrets were inoculated with 1 × 10⁵ TCID₅₀ of Pan/99wt and 1 × 10⁵ TCID₅₀ of Pan/99var viruses, as indicated in the legend to Fig. 5. Throat swabs were collected and virus therein analyzed to generate the data shown. (A) Viral genotypes of 17 to 21 plaque isolates per sample are shown in table format, as described in the legend to Fig. 1. Red bars indicate segments derived from the Pan/99wt virus, and turquoise bars show segments derived from the Pan/99var virus. White bars indicate segments where the genotyping result was not clear. (B) Infectious titers of throat swab samples are plotted as a function of time postinoculation. (C) The diversity of viral gene constellations detected is represented for each ferret, with days p.i. in grayscale. Each sample's Simpson index value was converted to a corresponding Hill number (Hill's N₂) to derive its effective diversity. (D) Results of next-generation sequencing of the IAV PB2 segments present in swab samples are shown. The percentage of reads carrying wt or var sequence is indicated by the coloring of the bars. Limit of detection = 1%.