FIG 1.

Expression and subcellular localization of six nonstructural proteins from a GII.4 HuNV strain. (A) Diagram of the six nonstructural proteins, including Nterm, NTPase, P22, VPg, Pro, and RdRp, within norovirus ORF1. The positions of proteolytic cleavage sites in the polyprotein precursor are indicated at the top of the diagram. (B) Western blot analysis of the viral nonstructural proteins expressed individually in 293T cells. The N-terminal FLAG-tagging expression plasmids that encode Nterm, NTPase, P22, VPg, Pro, or RdRp were transfected into 293T cells, and their protein expression was analyzed by immunoblotting using anti-FLAG antibody. CMV, cytomegalovirus. (C) Representative confocal microscopic images showing the subcellular localization of each nonstructural protein in A7 cells. Transfected cells were visualized using indirect immunofluorescence with anti-FLAG antibody. N, nucleus. Scale bars, 10 μm.