FIG 4.

C-terminal region of GII-NTPase is involved in mitochondrial targeting. (A) Diagram of the full length and deletion constructs of F-NTPase. The abilities of various F-NTPase constructs to form vesicles or to localize to mitochondria in the cell cytoplasm are summarized. The degrees of mitochondrial targeting of these F-NTPase constructs are indicated by a plus sign. (B) Western blot analysis of the indicated F-NTPase constructs expressed in 293T cells. (C) Representative confocal microscopic images showing the localization of various F-NTPase constructs and MitoTracker signals in A7 cells. White arrows indicate vesicular induction by F-NTPase or its deletion mutants in the cell. It should be noted that several N-terminal deletion constructs, including NTP(96–366), NTP(213–366), NTP(255–366), and NTP(96–271), showed high degrees of colocalization with the signals of MitoTracker. Scale bars, 10 μm.