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Rescue of CD63 increases vesicle secretion of LMP1 and mTOR-associated proteins. GFP-LMP1 and fused CD63-BirA proteins were transfected into HEK293 control or CD63 CRISPR cells. Cell lysates and EVs were collected for immunoblot analysis of mTOR-associated protein levels 24 h after transfection. All blots are representative images from independent experiments.
