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. 2018 Feb 12;92(5):e02097-17. doi: 10.1128/JVI.02097-17

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Copyright © 2018 Turkington et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Bat FLUAV NS1 proteins do not show a species-specific interaction with p85β. (A) 293T cells were cotransfected for 48 h with plasmids expressing the indicated V5-tagged NS1 protein (or GST) and FLAG-tagged human (Homo sapiens, H.s.) or bat (Myotis lucifugus, M.l.) p85β. After cell lysis, clarification, and anti-V5 immunoprecipitation, soluble (input) and pulldown (IP:α-V5) fractions were analyzed by SDS-PAGE and Western blotting. (B) Sequence alignment of iSH2 domains from human and bat p85β. Human (H. sapiens), little yellow shouldered bat (S. lilium), little brown bat (M. lucifugus), and Seba's short-tailed bat (C. perspicillata) p85β iSH2 domain amino acid sequences (residues 496 to 614) were aligned. Human/bat differences are highlighted by boxes, and shading indicates residues differing between bat species. (C) Experiment performed as in panel A, except using the indicated bat p85β mutants. For panels A and C, the data are representative of three independent experiments.