FIG 2.
FIP1C560–649 sequesters Env in a perinuclear compartment in a CT-dependent manner. (A) Wild-type GFP-FIP1C subcellular distribution in HeLa cells when expressed alone. (B) Subcellular distribution of GFP-FIP1C560–649. (C) Distribution of wild-type GFP-FIP1C when coexpressed with NL4-3 proviral DNA. Cells were fixed and immunolabeled with human neutralizing antibody 2G12 to stain HIV-1 gp120. Green, GFP-FIP1C; red, Env; rightmost image, overlay. (D) Distribution of FIP1C560–649 when coexpressed with NL4-3 proviral DNA. Staining is as described for panel C. (E) Coexpression of FIP1C560–649 and CT144 proviral plasmids stained and imaged as described for panel C. (F) GFP-FIP2452–512 and pNL4-3 proviral expression stained and imaged as described for panel C. All panels shown are from transfected HeLa cells. Images selected are representative of the major phenotypes found for more than 100 cells examined in repeated, independent experiments. Bars represent 10 μm.