FIG 5.

Effects of titration of FIP1C_560–649 on Env particle incorporation for Env CT trafficking mutants. (A) FIP1C_560–649 was cotransfected with the indicated proviral constructs at increasing amounts of DNA as indicated above the blots. Shown are Western blots for Env and Gag content in particles (top) and cell lysates (bottom). WT NL4-3 is shown as in Fig. 4 and serves as a control for inhibition of Env incorporation into particles. Note that S5 is the Env CT mutant YW₇₉₅SL, while S5R (revertant) is the second-site revertant in the CT, YW₇₉₅SL/L₈₅₀S. (B) Dose titration of FIP1C_560–649 as described for panel A, showing effects on CT144 and Y₇₁₂C Env compared to wild-type NL4-3. Particle blots are shown on the top, and cell lysates are below. Results are from HeLa cells.