FIG 7.
Dose titration of FIP1C560–649 and effects on SIVmac239 Env incorporation. (A) Comparison of effects of titration of FIP1C560–649 on Env incorporation by NL4-3 versus SIVmac239 in HeLa cells. In this experiment, full-length SIVmac239 proviral DNA was cotransfected with increasing amounts of FIP1C560–649. (B) CT-dependent reduction in Env incorporation illustrated by SIV-HIV Env chimera. (Left) Codon-optimized HIV-1 (JR-FL) Env was coexpressed with codon-optimized HIV Gag and increasing amounts of FIP1C560–649 DNA. (Middle) Codon-optimized SIVmac239 Env coexpressed with optimized SIVmac239 Gag and increasing amounts of FIP1C560–649. (Right) Effects of FIP1C560–649 expression on particle incorporation of a chimeric Env where the ectodomain and transmembrane domain of SIVmac239 Env was fused to the CT of HIV-1 Env. In this experiment, the Gag protein expressed was SIVmac239 Gag.