FIG 2.

Binding antibody multiplex assay (BAMA) analysis of rabbit immune serum reactivity to a global panel of HIV-1 gp120, gp140, and gp70-V1V2 scaffolds. Serial dilutions of sera from rabbits immunized with gp120 (4×) or cycP-gp120 (2×) were reacted against a multiclade panel of gp120/gp140 (A) and gp70-V1V2 (B) scaffold proteins and detected by a BAMA. Data are reported as AUC analysis values. Individual animal data points are plotted over box-and-whiskers plots. Combined AUC values were measured by calculating the average AUC for each animal against all gp70-V1V2 scaffold proteins. Shaded area represents threshold for positive reactivity. (C) Correlation analysis of gp70-V1V2 combined AUC of each immunized animal with their respective anti-JRFL endpoint titers (Fig. 1C). *, P < 0.05 by Mann-Whitney test and Spearman correlation test.