FIG 3.

V2-directed antibodies generated by cycP-gp120. (A) Alignment of V2 regions of cycP-gp120 and C.1086 gp120. V2 hot spot is indicated in red. (B) Overlapping 15-mer peptides derived from C.1086 were reacted against sera from rabbits immunized with gp120 (4×) or cycP (2×), and antibody binding was detected by ELISA. Lines represent individual animals, and animal numbers are indicated. (C) AUC analysis of binding to peptide 3. (D) Correlation analysis of gp70-V1V2 1086C scaffold binding (Fig. 2B) for each cycP-gp120-immunized animal with their respective binding to the indicated V2 peptide. Rabbits 29, 30, and 31 are matched against peptide 3. Rabbit 32 is matched against peptide 4. (E) V2 hot-spot binding to peptides derived from different HIV-1 strains, detected by ELISA binding and measured by OD₄₅₀. (F) Sequences of V2 hot-spot peptides used for panel E. *, P < 0.05 by Mann-Whitney test and Spearman correlation test.