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. 2018 Feb 12;92(5):e01606-17. doi: 10.1128/JVI.01606-17

FIG 6.

FIG 6

SF3 motif A is critical for the helicase activity of NV NS3. (A) Amino acid sequence alignment of NV NS3, SHV NS3, and MNV NS3. The SF3 consensus motifs A, B, and C are indicated. Completely conserved residues (100% identity) are highlighted in black. (B) The standard RNA helix (R*/R) substrate (0.1 pmol) was reacted with 20 pmol WT (lane 4), GK168AA (lane 5), or GK168AA-DD212AA (lane 6) MBP-NS3. Nonboiled reaction mixture (lane 1) and reaction mixture with addition of MBP alone (lane 3) were used as negative controls; the boiled reaction mixture (lane 2) was used as a positive control. (C) The standard RNA helix (R*/R) substrate (0.1 pmol) was reacted with 20 pmol GK168AA mutant MBP-NS3 in the presence of the indicated concentrations of ATP. (B and C) The asterisks indicate the HEX-labeled strands.