FIG 4.

Fold changes in gene expression between H. annosum 94233 and 94233/32D and H. parviporum RK15A* and RK15A. Detailed information is reported in Table 1 and in Table S3 in the supplemental material. RNA-Seq relative expression relates to log₂ fold change of reads per kilobase of transcript per million mapped reads (RPKM). RT-qPCR relative expression corresponds to log₂ fold change of expression ratios based on the take-off values normalized with three reference genes. Target genes 17 and 21 did not yield any amplification products from H. annosum 94233, and therefore the fold change could not be determined by RT-qPCR. Genes 1, 8, 13 to 15, and 23 were related to carbohydrate metabolism; genes 2, 4, 5, 11, and 26 were related to redox reactions and detoxification; genes 3 and 25 were related o cell wall and membrane related processes; genes 16, 17, and 24 were related to cell cycle control; gene 10 was related to inorganic metabolism; genes 6 and 7 were related to amino acid metabolism; gene 9 was related to mitochondrial energy production; genes 18, 19, and 27 were related to RNA-related processes; genes 22 and 28 were related to transcription, and genes 12 and 21 were related to sex.