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. 2018 Feb 12;92(5):e01744-17. doi: 10.1128/JVI.01744-17

TABLE 2.

Virus transmission experimentsa

Strain Species Collection site in Finland Host tree Collector(s) Yr HetPV13-an1 transmission Genotypeb Growth debilitationc
04120 H. annosum sensu stricto Myrskylä Picea abies K. Korhonen 2004 + n/RAMS Yes
07001 H. annosum sensu stricto Leppävirta Pinus sylvestris T. Hynönen 2007 2n NA
93177 H. annosum sensu stricto Toivakka, Huikko Pinus sylvestris K. Korhonen 1993 + 2n/si ND
05008 H. annosum sensu stricto Dragsfjärd, Rosendal Pinus sylvestris J.-O. Granvik 2005 2n NA
S26-5 H. annosum sensu stricto Läyliäinen Pinus sylvestris T. Piri, H. Nuorteva 2006 2n NA
S49-5 H. annosum sensu stricto Läyliäinen Pinus sylvestris T. Piri, H. Nuorteva 2006 + 2n/M13, RAMS No
00002 H. annosum sensu stricto Kirkkonummi, Solbacka Picea abies K. Korhonen 2000 2n NA
03019 H. annosum sensu stricto Västanfjärd, Nivelax Pinus sylvestris K. Korhonen 2003 + 2n/si ND
93173 H. annosum sensu stricto Liljendal Pinus sylvestris K. Korhonen 1993 + 2n/si ND
06066 H. annosum sensu stricto Alajärvi Pinus sylvestris A. Pajula 2006 + 2n/si No
03013 H. annosum sensu stricto Jämsänkoski Picea abies K. Korhonen 2003 + 2n/si No
02018 H. annosum sensu stricto Kisko, Toija Pinus sylvestris K. Lipponen, J. Aarnio 2002 + 2n/si No
93160 H. annosum sensu stricto Ruotsinpyhtää, Tesjoki Picea abies K. Korhonen 1993 + 2n/si No
RK15A H. parviporum Tuusula Picea abies E. Vainio, T. Piri 2010 + n/M, M13 Yes
RK5A H. parviporum Tuusula Picea abies E. Vainio, T. Piri 2010 2n NA
RK13A H. parviporum Tuusula Picea abies E. Vainio, T. Piri 2010 2n NA
5R182 H. parviporum Tuusula Picea abies T. Piri 2005 2n NA
RKU3.1.26 H. parviporum Tuusula Picea abies T. Piri 1994 2n NA
RKU3.2.34 H. parviporum Tuusula Picea abies T. Piri 1994 2n NA
LAP3.11 H. parviporum Lapinjärvi Picea abies T. Piri 1991 2n NA
15-05 H. parviporum Tuusula Picea abies T. Piri 2005 2n NA
73-05 H. parviporum Tuusula Picea abies T. Piri 2005 n NA
184-05 H. parviporum Tuusula Picea abies T. Piri 2005 n NA
109-05 H. parviporum Tuusula Picea abies T. Piri 2005 + n/M, M13 Yes
242-05d H. parviporum Tuusula Picea abies T. Piri 2005 + 2n/M13, RAMS Yes
a

The natural host of HetPV13-an1 is H. annosum 94233, isolated from Poland (20).

b

n, homokaryotic (haploid) recipient strain; 2n, = heterokaryotic (effectively diploid) recipient strain. Genome screening method after virus transmission: si, somatic incompatibility; M, mitochondrial markers; M13, M13 minisatellite fingerprinting; RAMS, = RAMS-PCR with primers CT, CCA, and CGA. In all cases, the genotype corresponded to the recipient strain.

c

NA, not applicable (no virus transmission detected); ND, = not determined.

d

This strain was cured of indigenous viruses by thermal treatment (consecutive incubations at 32 to 35°C for ∼10 weeks), and HetPV13-an1 was transmitted from H. parviporum RK15A.