TABLE 2.

Virus transmission experiments^a

Strain	Species	Collection site in Finland	Host tree	Collector(s)	Yr	HetPV13-an1 transmission	Genotypeb	Growth debilitationc
04120	H. annosum sensu stricto	Myrskylä	Picea abies	K. Korhonen	2004	+	n/RAMS	Yes
07001	H. annosum sensu stricto	Leppävirta	Pinus sylvestris	T. Hynönen	2007	−	2n	NA
93177	H. annosum sensu stricto	Toivakka, Huikko	Pinus sylvestris	K. Korhonen	1993	+	2n/si	ND
05008	H. annosum sensu stricto	Dragsfjärd, Rosendal	Pinus sylvestris	J.-O. Granvik	2005	−	2n	NA
S26-5	H. annosum sensu stricto	Läyliäinen	Pinus sylvestris	T. Piri, H. Nuorteva	2006	−	2n	NA
S49-5	H. annosum sensu stricto	Läyliäinen	Pinus sylvestris	T. Piri, H. Nuorteva	2006	+	2n/M13, RAMS	No
00002	H. annosum sensu stricto	Kirkkonummi, Solbacka	Picea abies	K. Korhonen	2000	−	2n	NA
03019	H. annosum sensu stricto	Västanfjärd, Nivelax	Pinus sylvestris	K. Korhonen	2003	+	2n/si	ND
93173	H. annosum sensu stricto	Liljendal	Pinus sylvestris	K. Korhonen	1993	+	2n/si	ND
06066	H. annosum sensu stricto	Alajärvi	Pinus sylvestris	A. Pajula	2006	+	2n/si	No
03013	H. annosum sensu stricto	Jämsänkoski	Picea abies	K. Korhonen	2003	+	2n/si	No
02018	H. annosum sensu stricto	Kisko, Toija	Pinus sylvestris	K. Lipponen, J. Aarnio	2002	+	2n/si	No
93160	H. annosum sensu stricto	Ruotsinpyhtää, Tesjoki	Picea abies	K. Korhonen	1993	+	2n/si	No
RK15A	H. parviporum	Tuusula	Picea abies	E. Vainio, T. Piri	2010	+	n/M, M13	Yes
RK5A	H. parviporum	Tuusula	Picea abies	E. Vainio, T. Piri	2010	−	2n	NA
RK13A	H. parviporum	Tuusula	Picea abies	E. Vainio, T. Piri	2010	−	2n	NA
5R182	H. parviporum	Tuusula	Picea abies	T. Piri	2005	−	2n	NA
RKU3.1.26	H. parviporum	Tuusula	Picea abies	T. Piri	1994	−	2n	NA
RKU3.2.34	H. parviporum	Tuusula	Picea abies	T. Piri	1994	−	2n	NA
LAP3.11	H. parviporum	Lapinjärvi	Picea abies	T. Piri	1991	−	2n	NA
15-05	H. parviporum	Tuusula	Picea abies	T. Piri	2005	−	2n	NA
73-05	H. parviporum	Tuusula	Picea abies	T. Piri	2005	−	n	NA
184-05	H. parviporum	Tuusula	Picea abies	T. Piri	2005	−	n	NA
109-05	H. parviporum	Tuusula	Picea abies	T. Piri	2005	+	n/M, M13	Yes
242-05d	H. parviporum	Tuusula	Picea abies	T. Piri	2005	+	2n/M13, RAMS	Yes

^aThe natural host of HetPV13-an1 is H. annosum 94233, isolated from Poland (20).

^bn, homokaryotic (haploid) recipient strain; 2n, = heterokaryotic (effectively diploid) recipient strain. Genome screening method after virus transmission: si, somatic incompatibility; M, mitochondrial markers; M13, M13 minisatellite fingerprinting; RAMS, = RAMS-PCR with primers CT, CCA, and CGA. In all cases, the genotype corresponded to the recipient strain.

^cNA, not applicable (no virus transmission detected); ND, = not determined.

^dThis strain was cured of indigenous viruses by thermal treatment (consecutive incubations at 32 to 35°C for ∼10 weeks), and HetPV13-an1 was transmitted from H. parviporum RK15A.