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Evolution in Nef195-203MW9 confers escape from CD8TL. (A) PBMC from animal r97088 8 weeks after SIV infection were tested for reactivity to dilutions of peptides representing the wild-type Nef195-203MW9 peptide (WT) and the most common variants identified by deep sequencing in our cohort. (B) Reactivity to variant peptides assessed with week 8 PBMC from three animals from our cohort, tested in duplicate IFN-γ ELISpot wells. Shown are the reactivities relative to the wild type.
