FIG 5.
Specific variants of Nef195-203MW9 compromise Tetherin and CD28 modulation but not CD3 or MHC-I. (A) Schematic showing how the downregulation of surface molecules was detected. (B) n-fold decrease in the MFI values of surface Tetherin expression in Gag p27+ cells relative to Gag p27− (uninfected) cells in the same samples. Cells from at least four RM were infected with each virus. (C to E) Same as in panel B but surface CD28 (C), CD3 (D), and MHC-I (E) levels were measured. Above each bar graph is a representative histogram showing the strategy for assessing surface downregulation of each molecule. For each tested molecule, the n-fold downregulation was compared between each variant and wild-type SIVmac239 using a paired t test (*, P < 0.05).