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Impact of mutations in the Nef195-203MW9 epitope on surface Envelope expression. (A) MFIs of surface Env expression in Gag p27+ cells infected with SIVmac239 and the indicated mutants. (B) n-fold increases in surface Envelope expression in cells infected with each mutant virus compared to cells infected with wild-type SIVmac239. *, P < 0.05 for each measure compared to cells infected with wild-type SIVmac239 tested using a two-tailed t test.
