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. 2018 Feb 13;18:56. doi: 10.1186/s12906-018-2115-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Annexin V/7AAD flowcytometry. MDA-MB-231 spheroids with low concentration (2.5 μg/mL) of citral, showed 8.99 ± 2.20% and 9.78 ± 2.10% for early and late apoptosis respectively, increasing the concentration to 5.0 μg/mL showed 38.41 ± 3.34% and 31.24 ± 3.14% and the high concentration (10.0 μg/mL) showed 43.28 ± 3.46% and 38.67 ± 2.77% for early and late apoptosis respectively compared to vehicle control that showed 1.19 ± 0.74% and 1.44 ± 0.82% of early and late apoptosis. All the experiments were performed in three biological replicates and each with triplicates. All data are expressed as mean ± S.E.M. with (*p < 0.05)