Fig. 7.

Cholesterol and control of PRV post-entry. MA104 cells (PRV) and BHK-21 cells (VSV) were first infected at 37 °C for 1 h then washed to remove extra virus. Cells were then treated with 8 mM MβCD incubated at 37 °C for 24 h. Antibodies to the PRV VP7 or VP4 proteins (PRV) and to the VSV G protein (VSV) were used to screen Western blots of cell homogenates. a Virus titer of PRV and VSV after treatment. b Western blot of PRV VP7 and VP4 proteins and β-actin. c Western blot of VSV G protein and β-actin. Western blots depict representative experiments. Error bars (Fig. 7a) indicate the standard deviations of three independent experiments