Fig. 8.

Reversing the effects of MβCD on PRV post-entry using exogenous cholesterol. MA104 cells (PRV) and BHK-21 cells (VSV) that had been infected and treated with MβCD were then incubated in the presence of 200 μM exogenous cholesterol. Western blots and virus titers were performed to assess the effects of pre- and post-treatment on virus infection. a Virus titer of PRV and VSV after treatment. b Western blot of PRV VP7 and VP4 proteins and β-actin. c Western blot of VSV G protein and β-actin. Western blots depict representative experiments. Error bars (Fig. 8a) indicate the standard deviations of three independent experiments