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. 2018 Feb 12;19:51. doi: 10.1186/s12859-018-2042-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Precision and Accuracy. a Precision of the distances between antigens on the map was determined using random sampling of HA sequences (0–4000 sequences) to construct the map. Each sampling was performed 50 times. Precision is given as the mean percent difference between distances of randomly chosen sequences and distances in original map. Error bars represent the standard deviation between results of sequence samplings. b ROC-AUC measurements of accuracy for each of assay type and criteria, HAI-A and HAI B represent 2-fold and 1:40-dilution criteria, respectively. c ROC curves over the range of antigenic distances cutoffs