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. 2018 Jan 5;18:5. doi: 10.1186/s12866-017-1147-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Identification of AnkX host targets by a NAPPA interaction assay. a Schematic illustration of the NAPPA-based protein-protein interaction screen. b NAPPA protein microarray after probing with HaloTag (control; left) or AnkX-HaloTag (right). Blue indicates no/low signal intensity, while red indicates maximal signal intensity. Inset shows a magnified view of the NAPPA regions containing the GST-PLEKHN1 spots (arrows) illuminated after incubation with the Alexa Fluor 660-conjugated HaloTag ligand. c List of AnkX host target candidates identified by NAPPA with a Z-score above 3