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. 2018 Jan 5;18:5. doi: 10.1186/s12866-017-1147-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — AnkX and PLEKHN1 co-localize on membrane compartments. a, b HEK293T cells were transiently transfected with plasmids encoding HaloTag-AnkX or -AnkX^H229A. HaloTag-TMR fluorescent ligand was utilized to visualize AnkX-fusion proteins, and anti-PLEKHN1 antibody to immunostain endogenous PLEKHN1. SR-SIM images displayed co-localization between PLEKHN1 and HaloTag-AnkX on vesicular structures (marked by yellow arrows). HaloTag-AnkX^H229A also co-localizes with PLEKHN1 on a similar structure. Upper and side panels show orthogonal views of the HEK293T cell expressing either HaloTag-AnkX or -AnkX^H229A confirming the intracellular location of the vesicular structure where co-localization was observed and highlighting accumulation of HaloTag-AnkX at the plasma membrane