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. 2018 Feb 8;8:32. doi: 10.3389/fcimb.2018.00032

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Copyright © 2018 Dai, He, Chang, Cao, Zhao, Huang, Wu, Huang, Yan, Han, Ma, Wen and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Natural transformation stability analysis of H. parasuis SC1401 after continuously passaging. The natural frequencies of every 10 generations of H. parasuis SC1401 were determined through TSB++ transformation methodology () and TSA++ transformation methodology (). The natural transformation frequency was determined from the number of antibiotic-resistant cfu mL⁻¹ divided by the total cfu mL⁻¹ scored on non-selective agar. Data points represent the mean values of three replicates, and error bars indicate standard deviations.

Inline graphic — Natural transformation stability analysis of H. parasuis SC1401 after continuously passaging. The natural frequencies of every 10 generations of H. parasuis SC1401 were determined through TSB++ transformation methodology () and TSA++ transformation methodology (). The natural transformation frequency was determined from the number of antibiotic-resistant cfu mL⁻¹ divided by the total cfu mL⁻¹ scored on non-selective agar. Data points represent the mean values of three replicates, and error bars indicate standard deviations.