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. 2018 Feb 8;8:32. doi: 10.3389/fcimb.2018.00032

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Copyright © 2018 Dai, He, Chang, Cao, Zhao, Huang, Wu, Huang, Yan, Han, Ma, Wen and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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H. parasuis transformation could be inhibited with unmarked homologous DNA. H. parasuis transformation could be inhibited by exogenous DNA and moderately inhibited by DNA from closely-related A. pleuropneumoniae. Increasing amounts (0, 10, 50, 100, 500, 1,000, and 2,000 ng) of competitive DNA from either homologous and heterologous strains were added to the mixture with constant amount (1 μg) of SC1401ΔhtrA::kan chromosomal DNA. Transformation frequency shows the number of Kan^R transformants per cfu of recipient cells. Data are representative of three independent experiments. Solid lines with different colors indicate source of competitor DNA.