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. 2018 Feb 8;8:32. doi: 10.3389/fcimb.2018.00032

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Copyright © 2018 Dai, He, Chang, Cao, Zhao, Huang, Wu, Huang, Yan, Han, Ma, Wen and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression levels of competence genes of bacteria cultured in TSB++ and on TSA++. (A) qRT-PCR analysis of mRNA levels of candidate competence genes in H. parasuis SC1401in different growth phase measured by optical density at 600 nm. (B) qRT-PCR analysis of mRNA levels of H. parasuis SC1401 cultured in TSB++ and on TSA++. (C) Volcano plot of gene express levels of bacteria cultured on TSA++ compared to that in TSB++. Horizontal dotted line indicates significance test result and vertical dotted lines indicate fold changes. The data represent means standard errors (n = 3), and error bars indicate standard deviations. Asterisks indicate statistical significance using one-way ANOVA (*P < 0.05).