Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| STRAINS | ||
| H. parasuis | ||
| SC1401 | Serotype 11 clinical isolate (Sichuan, China), highly transformable strain | Dai et al., 2016 |
| EP3 | Serotype 13 clinical isolate (Sichuan, China), transformable strain | He et al., 2016 |
| SH0165 | Serotype 5 clinical isolate (Hebei, China), non-transformable strain | Yue et al., 2009 |
| SC1401ΔhtrA::kan | SC1401 derivative, hphtrA deletion, kanamycin resistant | Zhang L. et al., 2016 |
| A. pleuropneumoniae | ||
| SC1516 | Serotype 7 clinical isolate | Li Y. et al., 2016 |
| H. influenzae | ||
| QHH | Clinic isolate (Shanghai, China) | Laboratory collection |
| Bacillus subtilis | ||
| WR | Clinic isolate (Sichuan, China) | Laboratory collection |
| S. pneumoniae | ||
| WZH | Clinic isolate (Sichuan, China) | Laboratory collection |
| E. coli ZY16 | Clinical isolate, enterotoxigenic E. coli | Laboratory collection |
| DH5α | FΦ80ΔlacZΔM15Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 | Laboratory collection |
| PLASMIDS | ||
| pMD-19T(simple) | AmpR, T-vector | Takara |
| pK18mobsacB | KanR; suicide and narrow-broad-host vector | Laboratory collection |
| pMDHK | AmpR, KanR; a 2153-bp fragment containing the motif of 5′-ACCGCTTGT and the ΔhtrA::kan cassette in pMD19-T | Zhang et al., 2015; Zhang L. et al., 2016 |
| pKBHK | KanR, a 2153-bp fragment containing motif of 5′-ACCGCTTGT and the ΔhtrA::kan cassette in pK18mobsacB | Zhang et al., 2015 |