Figure 5.

Five plant-derived compounds activate AmHsTRPA but not SiHsTRPA. Responses of SiHsTRPA and AmHsTRPA to β-citronellol, camphor, diallyl disulfide, nerol, and thujone were tested by calcium imaging. The mean value of fold change of Fura-2 ratio with error bar (± SEM) is shown for the compound-stimulated DsRed-positive (blue) and DsRed-negative (yellow) cells. The total numbers of SiHsTRPA- and AmHsTRPA-transfected cells analyzed were 73–153 and 71–103, respectively. **, Significant difference by one-tailed Welch’s t test (P < 0.001). The traces in extended data Fig. 5-1 indicate the changes of intracellular calcium level in SiHsTRPA- and AmHsTRPA-expressing cells by above five compounds.