Figure 7.

Responses of nine AmHsTRPA/SiHsTRPA chimeric channels to camphor, diallyl disulfide, and decanoic acid. A, Schematic representation of nine AmHsTRPA/SiHsTRPA chimeras as well as the parental AmHsTRPA and SiHsTRPA channels. Ankyrin repeat (AR) and transmembrane segment (TS) are shown by hexagon and rectangle, respectively. All chimeras were based on AmHsTRPA in which the N terminus (N-TER), AR1, AR2, AR3, AR3-5, AR5-7, the boundary between ARs and TS (AR/TS), the selectivity filter (SF), and the C terminus (C-TER) was swapped by the corresponding domain of SiHsTRPA. B, The mean value of fold change of Fura-2 ratio with error bar (± SEM) is shown for the compound-stimulated DsRed-positive (blue) and DsRed-negative (yellow) cells. The total numbers of camphor-, diallyl disulfide-, and decanoic acid-stimulated cells analyzed were 91–124, 87–122, and 180–246, respectively. **, Significant difference by one-tailed Welch’s t test (P < 0.001). The traces in extended data Fig. 7-1 and 7-2 indicate the changes of intracellular calcium level in cells expressing above AmHsTRPA/SiHsTRPA chimeric channel by camphor, diallyl disulfide, or C10. Protein expression of nine AmHsTRPA/SiHsTRPA chimeric channels is shown in extended data Fig. 7-3.