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. 2018 Feb 12;18:21. doi: 10.1186/s12862-018-1134-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Overexpression of LaoB C-terminally fused to EGFP. E. coli Top10 was transformed with the empty pEGFP vector as positive control (left) and with pEGFP-LaoB (right). Fluorescence values in logarithmic scale without (black) and with (white) induction are depicted. Expression of the fusion protein was induced with 10 mM IPTG after adjusting the OD₆₀₀ to 0.6. The experiment was performed in triplicate (*** p < 0.001)