Table 1.

Transcription and translation of laoB (part 1A) and its mother gene ECs5115 (part 1B) at the three different growth conditions indicated. The RPKM values of the transcriptome (RNAseq) and the translatome (RIBOseq) data for the overlapping novel gene and annotated mother gene are listed, including the RCV, indicating their translatability. ORF coverage is the fraction of a gene sequence, which is covered by RIBOseq reads. In addition, the corresponding data for the putative overlapping gene laoA (compare Fig. 2a) are shown (part 1C)

Condition	RPKM transcriptomea	RPKM translatomea	RCVa	ORF coveragea
1A laoB
LB, 37 °C	29.5	194	6.83	0.7
BHI, 37 °C	49.4	23.6	0.48	0.6
BHI + 4% NaCl, 14 °C	28.3	0.2	0.01	0.07
1B ECs5115
LB, 37 °C	19	12.3	0.65	0.35
BHI, 37 °C	26.9	7.1	0.27	0.45
BHI + 4% NaCl, 14 °C	10.2	0.6	0.07	0.16
1C laoA
LB, 37 °C	38.3	9.6	0.27	0.51
BHI, 37 °C	36.3	1.9	0.05	0.37
BHI + 4% NaCl, 14 °C	12.7	0.4	0.02	0.16

^aMean values of the two biological replicates are shown