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. Author manuscript; available in PMC: 2018 Mar 8.
Published in final edited form as: J Am Chem Soc. 2017 Feb 27;139(9):3446–3455. doi: 10.1021/jacs.6b11273

Figure 3.

Figure 3

Biological activity of Targapremir-210. (A) Expression of various RNAs in MDA-MB-231 cells under hypoxic conditions, showing increased levels of pri-, pre-, and mature miR-210 and HIF-1α and decreased levels of GPD1L. *, p< 0.05 compared to normoxic expression, as determined by a two tailed Student t test. (B) Effect of Targapremir-210 on levels of mature miR-210 in MDA-MB-231 cells cultured under hypoxic conditions, as determined by RT-qPCR *, p < 0.05 and **, p < 0.01 compared to untreated, as determined by a two-tailed Student t test. (C) Effect of Targapremir-210 (200 nM) and an antagomir directed against miR-210 (50 nM) on pri- and pre-miR-210, HIF-1α, and GPD1L levels in MDA-MB-231 cells as determined RT-qPCR The dashed line represents no change in RNA levels as compared to untreated MDA-MB-231 cells. *, p < 0.05 compared to untreated, as determined by a two-tailed Student t test.