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. 2018 Jan 18;41(4):1855–1866. doi: 10.3892/ijmm.2018.3405

Figure 3.

Figure 3

Effect of miR-21-5p antagomir on DOX sensitivity of SGC7901/DOX-2 μg/ml cells. (A) Transfection of anti-miR-21 decreased miR-21-5p expression levels in SGC7901/DOX-2 μg/ml cells to 44.8% of those in the anti-miR-NC group. (B) After transfection of SGC7901/DOX cells with anti-miR-21 or anti-miR-NC for 24 h and treatment with various doses of DOX for 48 h, the cell viability was assessed. (C) Flow cytometric analysis determined the influence of miR-21-5p on the apoptosis of SGC7901/DOX-2 μg/ml cells following treatment with DOX (1 μg/ml) for 48 h. The proportions of dead cells (Q1: Annexin V-FITC−/PI+), late apoptotic or necrotic cells (Q2: Annexin V-FITC+/PI+), early apoptotic cells (Q3: Annexin V-FITC+/PI−) and live cells (Q4: Annexin V-FITC−/PI−) are displayed. *P<0.05, **P<0.01 and ***P<0.001 vs. anti-miR-NC. miR, microRNA; FITC, fluorescein isothiocyanate; PI, propidium iodide; DOX, doxorubicin; SGC7901/DOX, DOX-resistant SGC7901 cell line; NC, negative control; Q, quadrant.