Table II.

Primers and other oligonucleotides sequences.

Name	Sequence (5′-3′)	Product length (bp)
miR-21-5p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCG CACTGGATACGACTCAACA
miR-21-5p-F	GCACCTAGCTTATCAGACTGA	22
miR-21-5p-R	GTGCAGGGTCCGAGGT
U6-F C	TCGCTTGGCAGCACATATACT	90
U6-R	ACGCTTCACGAATTTGCGTGTC
PTEN-3′UTR-F	GCTTTGGTGGTAGTACTCTGGTTGTTAAGCT AGGTATTTTGAGACTACTTCCCCATCAC	59
PTEN-3′UTR-R	GTGATGGGGAAGTAGTCTCAAAATACCTAGC TTAACAACCAGAGTACTACCACCAAAGC
MUT-PTEN-F	GCTTTGGTGGTAGTACTCTGGTTGTATTCGAT GGTATTTTGAGACTACTTCCCCATCAC	59
MUT-PTEN- R	GTGATGGGGAAGTAGTCTCAAAATACCATCG AATACAACCAGAGTACTACCACCAAAGC
TIMP3-3′UTR(1)-F	TTGCATATACCCACATGGGGACATAAGCTAA TTTTTTTACAGGACACAGAATTCTGT	59
TIMP3-3′UTR(1)-R	ACAGAATTCTGTGTCCTGTAAAAAAATTAGC TTATGTCCCCATGTGGGTATATGCAA
MUT-TIMP3(1)-F	TTGCATATACCCACATGGGGACAATTCGATAT TTTTTTACAGGACACAGAATTCTGT	59
MUT-TIMP3(1)-R	ACAGAATTCTGTGTCCTGTAAAAAAATATCG AATTGTCCCCATGTGGGTATATGCAA
TIMP3-3′UTR(2)-F C	GATGTCAGAGGGCGGTTTTGAGCTTTCTATA AGCTATAGCTTTGTTTATTTCACCCGT	59
TIMP3-3′UTR(2)-R	ACGGGTGAAATAAACAAAGCTATAGCTTATA GAAAGCTCAAAACCGCCCTCTGACATCG
MUT-TIMP3(2)-F C	GATGTCAGAGGGCGGTTTTGAGCTTTCTAA TTCGATTAGCTTTGTTTATTTCACCCGT	59
MUT-TIMP3(2)-R	ACGGGTGAAATAAACAAAGCTAATCGAATTA GAAAGCTCAAAACCGCCCTCTGACATCG
PTEN-F	GCCCAGACTGCATACGAT	294
PTEN-R CC	TAATCTATTTGCCATCAA
TIMP3-F	TACCTGCCTTGCTTTGTG	257
TIMP3-R C	ATCTGGGAAGAGTTAGTGTC
Pre-miR-21-5p-F C	GCGGATCCGCATGGCAACACCAGTCGA	42
Pre-miR-21-5p-R CC	GCTCGAGTTGTCAGACAGCCCATCGA
GAPDH-F	GCTGAACGGGAAGCTCACTG	169
GAPDH-R	GTGCTCAGTGTAGCCCAGGA

F, forward primer; R, reverse primer; RT, reverse transcription primer; PTEN, phosphatase and tensin homologue; TIMP3, tissue inhibitor of matrix metalloproteinases 3; miR, microRNA; MUT, mutated; UTR, untranslated region.