Table II.
Name | Sequence (5′-3′) | Product length (bp) |
---|---|---|
miR-21-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCG CACTGGATACGACTCAACA |
|
miR-21-5p-F | GCACCTAGCTTATCAGACTGA | 22 |
miR-21-5p-R | GTGCAGGGTCCGAGGT | |
U6-F C | TCGCTTGGCAGCACATATACT | 90 |
U6-R | ACGCTTCACGAATTTGCGTGTC | |
PTEN-3′UTR-F | GCTTTGGTGGTAGTACTCTGGTTGTTAAGCT AGGTATTTTGAGACTACTTCCCCATCAC |
59 |
PTEN-3′UTR-R | GTGATGGGGAAGTAGTCTCAAAATACCTAGC TTAACAACCAGAGTACTACCACCAAAGC |
|
MUT-PTEN-F | GCTTTGGTGGTAGTACTCTGGTTGTATTCGAT GGTATTTTGAGACTACTTCCCCATCAC |
59 |
MUT-PTEN- R | GTGATGGGGAAGTAGTCTCAAAATACCATCG AATACAACCAGAGTACTACCACCAAAGC |
|
TIMP3-3′UTR(1)-F | TTGCATATACCCACATGGGGACATAAGCTAA TTTTTTTACAGGACACAGAATTCTGT |
59 |
TIMP3-3′UTR(1)-R | ACAGAATTCTGTGTCCTGTAAAAAAATTAGC TTATGTCCCCATGTGGGTATATGCAA |
|
MUT-TIMP3(1)-F | TTGCATATACCCACATGGGGACAATTCGATAT TTTTTTACAGGACACAGAATTCTGT |
59 |
MUT-TIMP3(1)-R | ACAGAATTCTGTGTCCTGTAAAAAAATATCG AATTGTCCCCATGTGGGTATATGCAA |
|
TIMP3-3′UTR(2)-F C | GATGTCAGAGGGCGGTTTTGAGCTTTCTATA AGCTATAGCTTTGTTTATTTCACCCGT |
59 |
TIMP3-3′UTR(2)-R | ACGGGTGAAATAAACAAAGCTATAGCTTATA GAAAGCTCAAAACCGCCCTCTGACATCG |
|
MUT-TIMP3(2)-F C | GATGTCAGAGGGCGGTTTTGAGCTTTCTAA TTCGATTAGCTTTGTTTATTTCACCCGT |
59 |
MUT-TIMP3(2)-R | ACGGGTGAAATAAACAAAGCTAATCGAATTA GAAAGCTCAAAACCGCCCTCTGACATCG |
|
PTEN-F | GCCCAGACTGCATACGAT | 294 |
PTEN-R CC | TAATCTATTTGCCATCAA | |
TIMP3-F | TACCTGCCTTGCTTTGTG | 257 |
TIMP3-R C | ATCTGGGAAGAGTTAGTGTC | |
Pre-miR-21-5p-F C | GCGGATCCGCATGGCAACACCAGTCGA | 42 |
Pre-miR-21-5p-R CC | GCTCGAGTTGTCAGACAGCCCATCGA | |
GAPDH-F | GCTGAACGGGAAGCTCACTG | 169 |
GAPDH-R | GTGCTCAGTGTAGCCCAGGA |
F, forward primer; R, reverse primer; RT, reverse transcription primer; PTEN, phosphatase and tensin homologue; TIMP3, tissue inhibitor of matrix metalloproteinases 3; miR, microRNA; MUT, mutated; UTR, untranslated region.