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. 2018 Jan 25;41(4):1976–1982. doi: 10.3892/ijmm.2018.3424

Figure 3.

Figure 3

(A) Western blot analysis of ICAM-1, GAPDH, p-p38 MAPK and total p38 MAPK in MSCs, EPCs and MSCs + EPCs, with or without IL-1β (25 µg/ml) or p38 MAPK inhibitor SB203580 (20 µmol/ml) treatment. Lanes 1–3, normally cultured MSCs, EPCs and MSCs + EPCs groups, respectively; lanes 4–6, IL-1β (25 µg/ml)-stimulated MSCs, EPCs and MSCs + EPCs groups, respectively; lanes 7–9, p38 MAPK inhibitor SB203580 (20 µmol/ml)-stimulated MSCs, EPCs and MSCs + EPCs groups, respectively. (B) ICAM-1/GAPDH and (C) p-p38 MAPK/total-p38 MAPK IOD relative values were determined. Data are presented as the means ± standard deviation of three independent experiments. *P<0.05. EPCs, endothelial progenitor cells; ICAM-1, intercellular adhesion molecule-1; IL-1β, interleukin-1β; IOD, integrated optical density; MAPK, mitogen-activated protein kinase; MSCs, mesenchymal stem cells.