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. 2018 Jan 15;41(4):1967–1975. doi: 10.3892/ijmm.2018.3388

Figure 4.

Figure 4

Cooperative functions of combined Let-7c and tamoxifen require ER and suppress Wnt signaling. (A) Let-7c overexpression in mammospheres reduced the expression of ERα and the key factors of Wnt signaling. (B) Tamoxifen functioned the manner as Let-7c with weaker suppressive effect. (C) The wild-type TCF-4 responsive luciferase promoter reporter assay was performed by using pCMV-Green Renilla luciferase assay and Pierce Luciferase Cell Lysis Buffer, and the activity was normalized to the Renilla luciferase activity. (D) Combined Let-7c and tamoxifen inhibited the Wnt signaling activity, however, the individual application of either Let-7c or tamoxifen failed to. TCF-4, T cell factor-4; ER, estrogen receptor; Oct-4, POU domain class 5 transcription factor 1; Sox-2, transcription factor SOX-2 (n=3 repeats with similar results. Values are presented as the means ± SEM).