Figure 5.

In vivo model of stem-like cells' formation of tumor and the effects of Let-7c on blocking the Wnt activity in tumor growth. (A) Tumor growth curve and tumor weight calculation revealed that Let-7c overexpression of stem-like cells significantly inhibited tumor growth in vivo (Scr, n=5; Let-7c, n=5). Scale bar, 1 cm). (B) Expression level of ERα and Wnt signaling factors in tumors arising from Let-7c overexpressed group was significantly lower than that arising from miR-control group, as determined by reverse transcription-quantitative polymerase chain reaction. (C) Representative images of Let-7c estrogen receptor and Wnt signaling factors in tumor tissues (n=3 repeats with similar results; magnification, ×400). Statistical significance was evaluated by using Kruskal-Wallis test or analysis of variance. ^*P<0.05 vs. Scr. Scr, scramble; ER, estrogen receptor; TCF-4, T cell factor. Values are presented as the means ± SEM.