Table I.
Primers used for polymerase chain reaction.
| GenBank ID | Gene name | Sequence | Annealing temperature (°C) | Length (bp) |
|---|---|---|---|---|
| GAPDH | F: 5′-GGGAAACTGTGGCGTGAT-3′ | 60 | 299 | |
| R: 5′-GAGTGGGTGTCGCTGTTGA-3′ | ||||
| NM_198721 | COL25A1 | F: 5′-CCTACACCACCCATTGAAAC-3′ | 60 | 184 |
| R: 5′-TCTCCGCCACCTTATCTCT-3′ | ||||
| NM_020987 | ANK3 | F: 5′-TTTATCAGCCAGTCCCAGTT-3′ | 60 | 62 |
| R: 5′-TTGTTCATCGTCCACTTCC-3′ | ||||
| NR_046402 | POLD1 | F: 5′-TTCATTCCAGTCCAAGCAGA-3′ | 60 | 127 |
| R: 5′-GGCATTGAGCGTGTAGGAG-3′ | ||||
| NM_024719 | GRTP1 | F: 5′-CCAACTTTCACTGCTTTCCT-3′ | 60 | 198 |
| R: 5′-ACAAACCCACACTCACATTTT-3′ | ||||
| NR_046583 | COL4A2-AS1 | F: 5′-CCTTTTGTGACTGGCTTCTT-3′ | 60 | 104 |
| R: 5′-CCAGCCTTTATTCGGTCTT-3′ | ||||
| NM_000689 | ALDH1A1 | F: 5′-ATTTCCCGTTGGTTATGCT-3′ | 60 | 86 |
| R: 5′-AGTTTGCTCTGCTGGTTTG-3′ | ||||
| ENST00000559026 | RP11-59H7.3 | F: 5′-TCAAAAGGCTAGAAATTGTGC-3′ | 60 | 131 |
| R: 5′-CTCCAAAACTGCTGAAACTACA-3′ | ||||
| NR_125715 | TGFB2-OT1 | F: 5′-CTCATGCTGGGGTTAATAGAA-3′ | 60 | 114 |
| R: 5′-GCTGCTCTTTAGGTGAAACTG-3′ |
F, forward; R, reverse; TGFB2-OT1, transforming growth factor β2 overlapping transcript 1; POLD1, DNA polymerase δ1, catalytic subunit; COL25A1, collagen type XXV alpha 1 chain; ANK3, ankyrin 3; GRTP1, growth hormone regulated TBC protein 1; COL4A2-AS1, COL4A2 antisense RNA 1; ALDH1A1, aldehyde dehydrogenase 1 family member A1.