Figure 1.

miR-647 expression is decreased in GC tissue specimens and drug-resistant GC cells, and can overcome drug resistance in GC cells. (A) miR-647 expression in GC tissue specimens from patients with distant metastasis and adjacent nontumor tissues was detected by RT-qPCR (n=4). ^*P<0.05 vs. adjacent nontumor tissues. (B) miR-647 expression in SGC7901/VCR, SGC7901 and GES-1 cells was detected by RT-qPCR (n=4).^**P<0.05 vs. GES-1 cells; ^***P<0.05 vs. SGC7901 and GES-1 cells. (C) miR-647 expression was analyzed by RT-qPCR following infection (n=4). (D) IC₅₀ values for vincristine were calculated (n=4). ****P<0.05 vs. LV-GFP and control groups. Data are presented as the means ± standard error of the mean. GC, gastric cancer; GFP, green fluorescent protein; IC₅₀, half maximal inhibitory concentration; miR-647, microRNA-647; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.