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. 2018 Jan 25;41(4):1949–1957. doi: 10.3892/ijmm.2018.3425

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Copyright: © Zheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Results of RIP and ChIP assays for primary HDFs. (A) Relative expression of EZH2 in heat-treated HDFs. (B) RIP analysis was performed using antibodies against EZH2 and IgG. (C) pc-EZH2 and si-EZH2 were transfected into HDFs. (D) ChIP analysis was performed using antibodies against H3K27me3 and IgG. (E) Relative expression of LET in HDFs transfected with pc-EZH2 or si-EZH2. (F) Correlation of LET and EZH2 expression in burn tissues from patients. ^*P<0.05 and ^**P<0.01 vs. control or input, respectively. RIP, RNA immunoprecipitation; ChIP, chromatin immunoprecipitation; HDF, human dermal fibroblast; EZH2, enhancer of zeste homolog 2; IgG, immunoglobulin G; pc-EZH2, EZH2 overexpression plasmid; si-EZH2, EZH2, small interfering RNA; LET, low expression in tumor; lncRNA, long non-coding RNA; H3K27me3, histone H3 lysine 27 trimethylation.