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. 2018 Jan 17;41(4):2086–2098. doi: 10.3892/ijmm.2018.3393

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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mRNA and protein expression levels of BCL2, BCL-xL and BAX in the NS natural aging group and D-gal-induced mimetic aging group. (Aa-c) Reverse transcription-quantitative polymerase chain reaction and (Ad-f) western blotting were used to study the mRNA and protein expression levels in the D-gal group and NS group, respectively. β-actin was used as a loading control. (B) Visualization of changes of BCL2 protein expression with aging was demonstrated using immunofluorescence, and the nuclei were stained with DAPI. Data are expressed as mean ± standard error of the mean. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. the age-matched NS subgroup; ^P<0.01 vs. the 3-month-old NS subgroup; ^#P<0.001 vs. the 3-month-old D-gal subgroup. Scale bar, 100 mm at magnification, ×200 and 50 mm at magnification, ×600. NS, normal saline; D-gal, D-galactose; BCL2, B-cell lymphoma 2; BCL-xL, B-cell lymphoma-extra large; BAX, B-cell lymphoma 2-associated X protein.

mRNA and protein expression levels of BCL2, BCL-xL and BAX in the NS natural aging group and D-gal-induced mimetic aging group. (Aa-c) Reverse transcription-quantitative polymerase chain reaction and (Ad-f) western blotting were used to study the mRNA and protein expression levels in the D-gal group and NS group, respectively. β-actin was used as a loading control. (B) Visualization of changes of BCL2 protein expression with aging was demonstrated using immunofluorescence, and the nuclei were stained with DAPI. Data are expressed as mean ± standard error of the mean. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. the age-matched NS subgroup; ^P<0.01 vs. the 3-month-old NS subgroup; ^#P<0.001 vs. the 3-month-old D-gal subgroup. Scale bar, 100 mm at magnification, ×200 and 50 mm at magnification, ×600. NS, normal saline; D-gal, D-galactose; BCL2, B-cell lymphoma 2; BCL-xL, B-cell lymphoma-extra large; BAX, B-cell lymphoma 2-associated X protein.